Device

Part:BBa_K561004:Design

Designed by: LI Ye, XIA Yu   Group: iGEM11_ZJU-China   (2011-10-03)

PfdhF+cex+cenA+tetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 649
    Illegal NotI site found at 1646
    Illegal NotI site found at 2790
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2703
    Illegal BamHI site found at 1839
    Illegal XhoI site found at 2201
    Illegal XhoI site found at 2450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 282
    Illegal NgoMIV site found at 655
    Illegal NgoMIV site found at 1157
    Illegal NgoMIV site found at 1981
    Illegal NgoMIV site found at 2906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1885
    Illegal BsaI.rc site found at 702
    Illegal SapI.rc site found at 785


Design Notes

This device has strong RBS. Cex and CenA are expressed in the same time for the efficient degradation of cellulose into cellobiose.


Source

PfdhF comes from E.coli DH5α genome.

References